In eucaryotic cells, DNA, the support of genetic information is wrapped in a complex nucleo-proteic structure called chromatin which dynamics plays an essential role in the interpretation of genetic information. This structure is regulated by several chromatin remodeling and modifying complexes and constitutes a second level of information so-called epigenetic information. From the last few years it has become clear that many cancers originate in incorrect establishment and/or mis-interpretation of this epigenetic information opening possibilities for new therapeutic strategies.
In the laboratory we are interested in studying the epigenetic mechanisms that regulate the choice between cell proliferation and differentiation. Our researches are focused on two families of proteins: HP1 (Heterochromatin protein 1) and TIF1 (Transcriptional Intermediary Factor 1) with a particular interest in the corepressor TIF1β (also known as KAP1 or TRIM28). These proteins are molecular platforms that allow the recruitment of multiple factors implicated in chromatin dynamics and are involved in many cellular processes including gene expression regulation, DNA repair and more globally chromatin sub-nuclear organization. We showed in the laboratory that these proteins are essential in numerous physiological processes including embryonic development and cell differentiation as well as in several pathological processes such as the establishment and/or progression of some cancers. Our research is centered on the functional characterization of these two families of proteins at the chromatin level and understanding how these proteins influence cell homeostasis.
Fasching L, Kapopoulou A, Sachdeva R, Petri R, Jönsson ME, Männe C, Turelli P, Jern P, Cammas F, Trono D, Jakobsson J. TRIM28 represses transcription of endogenous retroviruses in neural progenitor cells. Cell Rep. 2015 Jan 6;10(1):20-8. doi: 10.1016/j. celrep .2014.12.004. Epub 2014 Dec 24.
Paul C, Sardet C, Fabbrizio E. The Wnt-target gene Dlk-1 is regulated by the Prmt5-associated factor Copr5 during adipogenic conversion. Biol Open. 2015 Feb 13;4(3):312-6. doi: 10.1242/bio. 201411247.
Harouz H, Rachez C, Meijer BM, Marteyn B, Donnadieu F, Cammas F, Muchardt C, Sansonetti P, Arbibe L. Shigella flexneri targets the HP1γ subcode through the phosphothreonine lyase OspF. EMBO J. 2014 Sep 12. pii: e201489244. [Epub ahead of print]
Herquel B, Ouararhni K, Martianov I, Le Gras S, Ye T, Keime C, Lerouge T, Jost B, Cammas F, Losson R, Davidson I. Trim24-repressed VL30 retrotransposons regulate gene expression by producing noncoding RNA. Nat Struct Mol Biol. 2013 Feb 3;20(3):339-46. doi: 10. 10 38 /n s m b.2496. Epub 2013 Feb 3.
Allan RS, Zueva E, Cammas F, Schreiber HA, Masson V, Belz GT, Roche D, Maison C, Quivy JP, Almouzni G, Amigorena S. An epigenetic silencing pathway controlling T helper 2 cell lineage commitment. Nature. 2012 Jul 12;487(7406):249-53